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In chick embryos, the neurogenic region of the somite is inverted by injecting the animal pole of the embryo with a DNA vector encoding a nuclear localized GFP (m-GFP). The inversion is a bilateral and symmetrical process, producing a mirror image of the somitic mesoderm. The ventral half of the somitic mesoderm is inversed by the injection of the presumptive ventral neural tube in a 0-2-hss after the onset of somitogenesis. The dorsal half of the somitic mesoderm is inversed by the injection of the presumptive dorsal neural tube in a 2-4-hss after the onset of somitogenesis. In both cases, the inversion is completed by a caudal migration of the injected cells to the appropriate side of the axis. The dorsal half of the somitic mesoderm inversed by the injection of the dorsal neural tube is characterized by a decrease in the number of neurons in the intermediate zone of the spinal cord. The ventral half of the somitic mesoderm inversed by the injection of the ventral neural tube is characterized by a decrease in the density of neurons in the medial part of the intermediate zone. The cell death is not restricted to the intermediate zone, but the neurons of the ventral spinal cord are much less affected. The injection of m-GFP in the intermediate zone of the dorsal part of the somitic mesoderm inversed by the injection of the dorsal neural tube induces a more intense cell death than the injection of m-GFP in the ventral part of the somitic mesoderm inversed by the injection of the ventral neural tube. The cell death appears to be restricted to the intermediate zone of the spinal cord.
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